Positron Emission Tomography Imaging of Hepatocellular Carcinoma with Radiolabeled Choline

 
 
 
 

Positron Emission Tomography Imaging of Hepatocellular Carcinoma with Radiolabeled Choline

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dc.contributor.advisor Lee, Zhenghong en_US
dc.contributor.author Kuang, Yu en_US
dc.date.accessioned 2009-04-09T22:23:52Z
dc.date.available 2009-04-09T22:23:52Z
dc.date.created 2009 en_US
dc.date.issued 2009-04-09T22:23:52Z
dc.identifier.uri http://rave.ohiolink.edu/etdc/view?acc_num=case1238781224 en_US
dc.identifier.uri http://hdl.handle.net/2374.OX/104241
dc.description Hepatocellular Carcinoma (HCC) is one of the most common malignancies throughout the world and its five-year survival rate has been dismal (5%). The carcinogenesis is frequently associated with the metabolic changes that precede the morphological changes. Therefore, a non-invasive, fast, quantitative technique for detection of HCC is much needed. Positron emission tomography (PET), a molecular imaging technique, holds particular promise for diagnostic imaging of neoplasms. The focus of this thesis is on the diagnostic utility of PET metabolic imaging on HCC and the mechanisms underlying the imaging using radiolabeled choline (CHOL) as the tracer. (1) Many cancers display a high rate of aerobic glycolysis, a phenomenon that is exploited by 2-Deoxy-2-[^18F]-fluoro-D-glucose (FDG) PET imaging for the detection of tumors. Up-regulation of glycolytic metabolism plays a role in tumor progression by contributing to tumor growth or survival. In this study, the usefulness of FDG-PET for HCC was investigated. The study addressed the correlation between FDG-PET images with pathologic types of HCC. The overall sensitivity of FDG-PET in the detection of HCC is low (50-55%). This can be explained by the wide variability in enzyme activity in the individual HCC. In well-differentiated HCC, FDG metabolism may be similar to that of the surrounding liver, leading to a false negative result, while higher sensitivity was reported in poorly differentiated HCC. (2) Increased lipid synthesis is required by a growing tumor cell to synthesize membranes and lipid-modified signaling molecules. The radiolabeled choline (CHOL) was used to probe lipid synthesis in HCC. In this study, PET/CT imaging was correlated with metabolites analysis in vivo and in vitro, which helps to explain the heterogenous uptake of radiolabeled CHOL in HCC. Transport and phosphorylation of CHOL are responsible for the tracer accumulation during [^11C]-CHOL PET imaging in well-differentiated HCC. Moreover, basal oxidation and phosphorylation activities in surrounding hepatic tissue contribute to the background signal seen in [^11C]-CHOL PET images. Furthermore, PET imaging of lipid synthesis with radiolabeled CHOL is useful in well-differentiated HCC that is not FDG avid. PET/CT imaging with radiolabeled CHOL could thus be a very promising diagnostic tool in patients with suspicious liver masses. en_US
dc.format application/pdf en_US
dc.format 226p. en_US
dc.rights restricted; full text unavailable until 2009-10-03 en_US
dc.rights Copyright and permissions information available at the source archive en_US
dc.subject Hepatocellular Carcinoma en_US
dc.subject Positron Emission Tomography (PET) en_US
dc.subject [^11C]-choline en_US
dc.subject Molecular imaging en_US
dc.subject FDG en_US
dc.subject Cancer Metabolism en_US
dc.subject Transport mechanism en_US
dc.subject Phosphatidylcholine synthesis en_US
dc.subject Lipids en_US
dc.subject High performance liquid chromatography en_US
dc.subject Thin layer chromatograph en_US
dc.title Positron Emission Tomography Imaging of Hepatocellular Carcinoma with Radiolabeled Choline en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name PhD en_US
dc.degree.level doctoral en_US
dc.degree.discipline Biomedical Engineering en_US
dc.degree.grantor Case Western Reserve University en_US
dc.contributor.publisher Case Western Reserve University / OhioLINK en_US

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