Ugene, a Newly Identified Protein that is Commonly Over-Expressed in Cancer, and that Binds to Uracil DNA-Glycosylase

Title:	Ugene, a Newly Identified Protein that is Commonly Over-Expressed in Cancer, and that Binds to Uracil DNA-Glycosylase
Author:	Guo, Chunguang
Description:	Expression microarrays identified a novel transcript, designated as Ugene, whose expression is absent in normal colon and colon adenomas, but that is commonly induced in malignant colon cancers. These findings were validated by real-time PCR and Northern blot analysis in an independent panel of colon cancer cases. In addition, Ugene expression was found to be elevated in many other common cancer types, including, breast, lung, uterus, and ovary. Immunofluorescence of V5-tagged Ugene revealed it to have a nuclear localization. In a pull-down assay, uracil DNA-glycosylase 2 (UNG2), an important enzyme in the base excision repair pathway, was identified as a partner protein that binds to Ugene. Co-immunoprecipitation and Western blot analysis confirmed the binding between the endogenous Ugene and UNG2 proteins. Using deletion constructs, we find that Ugene binds to the first 25 amino acids of the UNG2 NH2-terminus. We suggest Ugene induction in cancer may contribute to the cancer phenotype by interacting with the base excision repair pathway.
Permanent Link:	http://rave.ohiolink.edu/etdc/view?acc_num=case1217422102 http://hdl.handle.net/2374.OX/104324
Date:	2009

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