INVESTIGATIONS OF SEPARATION MECHANISMS IN CAPILLARY ELECTROPHORESIS AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

 
 
 
 

INVESTIGATIONS OF SEPARATION MECHANISMS IN CAPILLARY ELECTROPHORESIS AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

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Title: INVESTIGATIONS OF SEPARATION MECHANISMS IN CAPILLARY ELECTROPHORESIS AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Author: CABOVSKA, BAIBA
Description: In the beginning of this work, the applications of ionic liquids to chromatography and capillary electrophoresis are reviewed. This review discusses the interactions ionic liquids provide in the retention and separation of various analytes. The use of ionic liquids is further encompassed in Chapters 2, 3 and 4. Aqueous capillary electrophoretic studies were performed to investigate interactions between halophenols and 1-ethyl-3-methylimidazolium tetrafluoroborate or tetraethylammonium tetrafluoroborate electrolytes. In both cases, the impact of halogen size and place of substitution was investigated. Investigating the interactions between halophenols and ionic liquids by NMR, the NMR results indicated hydrophobic stacking interactions between the iodophenols and possible similar interaction among phenols and imidazolium. Following the CE studies, the applications of ionic liquids to HPLC were also investigated. Two different alkylimidazoles were attached to silica support and the effect of organic modifier, pH and ionic strength on retention was examined. The imidazolium based stationary phase was compared with a reversed phase column, which incorporated only alkylbromide as the functional group. The analytes selected allowed differentiation between reversed phase and ion-exchange phase behavior on the stationary phases. In Chapter 5, the separation of cocaine stereoisomers and metabolites was investigated. The effect of methanol and acetonitrile as organic modifiers and the effect of different concentrations of sulfated cyclodextrin on the resolution and migration times of the analytes were investigated. A reproducible method for the separation of cocaine stereoisomers was developed.
Permanent Link: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1064411271
http://hdl.handle.net/2374.OX/10474
Date: 2003

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