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ASSESSMENT OF METAL CONCENTRATIONS AND SPECIES IN BIOLOGICALLY RELEVANT SAMPLES WITH INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRIC TECHNIQUES
| Title: | ASSESSMENT OF METAL CONCENTRATIONS AND SPECIES IN BIOLOGICALLY RELEVANT SAMPLES WITH INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRIC TECHNIQUES |
| Author: | DeNicola Cafferky, Katie |
| Description: | For biological samples, total elemental analysis as well as elemental speciation is especially important. In this dissertation, inductively coupled plasma mass spectrometry is presented as a viable technique for the analysis of total metal content in a variety of sample types. Coupling this method with different chromatography methods allows the different species of these metals to be determined. The study of selenium in yeast has become increasingly important amidst reports of this elements effectiveness in cancer chemoprevention. S. cerevisiae is commonly used to make selenium supplements due to its ability to incorporate relative large levels of selenium into the biologically available form of selenomethionine (SeMet). The investigation of methods that increase this level of incorporation, which maintaining SeMet as the predominant species could be beneficial in the production of such supplements. SeCys, which is incorporated into yeast proteins by specific, rather than non-specific mechanism, is thought to be a biochemically superior form of selenium for cancer chemoprevention. Identifying this compound in specific yeast proteins would be useful in developing more effective antitumorogenic supplements. Metals are very important to the biological function of many organisms. It is expected that this would also be true for viruses, but no comprehensive study of the metal content of viruses has ever been conducted. Likewise, no investigation of cellular metal content during viral infection has ever been reported. By comparing the metal concentrations in healthy cells and HSV-1 infected cells, it is possible to identify elements that are involved or affected by the infection and replication of this virus. Similarly, the metals associated with cancer can be studied by the same techniques. Comparison of metal levels and species in cancerous and non-cancerous lung tissues could provide information useful in better understanding carcinogensesis. Lastly, arsenic contamination plagues many areas of Bangladesh. Evaluating the levels of this toxicant in cooked rice and vegetable samples from the region is important to fully measure the level of exposure. Determining the specific arsenic species in these samples is necessary to assess the true risk of such exposure. |
| Permanent Link: |
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1155754956
http://hdl.handle.net/2374.OX/11161 |
| Date: | 2006 |
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