PHOTOSYNTHETIC PICOPLANKTON AND BACTERIOPLANKTON IN THE CENTRAL BASIN OF LAKE ERIE DURING SEASONAL HYPOXIA

 
 
 
 

PHOTOSYNTHETIC PICOPLANKTON AND BACTERIOPLANKTON IN THE CENTRAL BASIN OF LAKE ERIE DURING SEASONAL HYPOXIA

Show full item record


Title: PHOTOSYNTHETIC PICOPLANKTON AND BACTERIOPLANKTON IN THE CENTRAL BASIN OF LAKE ERIE DURING SEASONAL HYPOXIA
Author: Cupp, Audrey R.
Description: In Lake Erie’s central basin, a hypoxic region commonly termed the dead zone forms during late summer. Previous work has demonstrated an abundance of photosynthetic picocyanobacteria despite this lack of oxygen. High-throughput sequencing of over 400 cyanobacterial and eubacterial 16S rDNA amplicons has characterized some of the major members of the microbial community both during and prior to the dead zone formation. In July, the bacterial communities mainly consisted of two unique clusters of Gram-positive Actinobacteria, with a smaller percentage of Flexibacter-Cytophaga-Bacteroides , α, β, and γ Proteobacteria . Cyanobacteria in the form of photosynthetic picoplankton was found at 16.5 m in July, but was absent from the 20 m library. During hypoxia in August, a community shift was demonstrated with a decrease in the Flexibacter-Cytophaga-Bacteroides , an increase in number and diversity of cyanobacteria, and an increase in an α-Proteobacterial cluster. Diurnal oxygen production in the hypolimnion of Lake Erie was exhibited by in situ probes and showed actively photosynthetic picoplankton producing oxygen. Cyanobacterial 16S libraries showed an increase in diversity of photosynthetic picoplankton in August compared to July. The vast majority of clones similar to Synechococcus sp. MH301 were found in July with only a small percentage of clones from other groups. Conversely, during hypoxia, an increase of diversity was shown to exist. These differences in bacterial community members indicate the cycling of oxygen may influence the community structure in Lake Erie. Novel primers specific for the cpeB gene in phyoerythrin-rich (PE-rich) cyanobacteria were used to study diversity of potential marine-like forms, indicating a new way to phylogenetically study PE-rich cyanobacteria.
Permanent Link: http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1150908860
http://hdl.handle.net/2374.OX/15434
Date: 2006

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show full item record