Identification of Francisella tularensis Outer Membrane Proteins

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Title:	Identification of Francisella tularensis Outer Membrane Proteins
Author:	Melillo, Amanda Adeline
Description:	Francisella tularensis is a Gram-negative bacterium that causes tularemia. It is a potential biological weapon because of its extreme infectivity (<10 organisms), ease of dissemination, and substantial capacity to cause illness and death. Little is known about the pathogenesis of F. tularensis infections. We hypothesize that adherence of F. tularensis to lung epithelium is an important first step in pathogenesis and that surface proteins (adhesins) are critical for this adherence. We have observed enhanced adherence of F. tularensis LVS (live vaccine strain) to the human epithelial cell line, A549, when compared to the adherence of subsp. novicida or laboratory strains of E. coli. To identify surface proteins, bacteria were treated with a membrane impermeable biotinylation agent. We have identified one known F. tularensis surface protein (FopA) and seven putative outer membrane proteins. This work will give us a greater understanding of the initial steps in F. tularensis pathogenesis.
Permanent Link:	http://rave.ohiolink.edu/etdc/view?acc_num=mco1121867713 http://hdl.handle.net/2374.OX/18122
Date:	2005

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Now showing item 84 of 175