Home
Tags
No Tags
EXAMINATION OF MITOCHONDRIAL CIT3 IN SACCHAROMYCES CEREVISIAE AS THE GENE FOR METHYLCITRATE SYNTHASE
| Title: | EXAMINATION OF MITOCHONDRIAL CIT3 IN SACCHAROMYCES CEREVISIAE AS THE GENE FOR METHYLCITRATE SYNTHASE |
| Author: | Graybill, Eric R. |
| Description: | The existence of a methylcitrate pathway for propionate metabolism in yeasts has been shown but the 2-methylcitrate synthase in this pathway had not been clearly identified. In this study, the product of the CIT3 gene has been identified as a dual specific citrate and methylcitrate synthase and that of the CIT1 gene as a specific citrate synthase. Cit1p has catalytic activity only with acetyl-CoA as substrate (kcat / Km = 150x103 M-1 s-1) whereas Cit3p has similar catalytic efficiencies with both acetyl-CoA (kcat / Km = 9x103 M-1 s-1) and propionyl-CoA (kcat / Km = 10x103 M-1 s-1). Deletion of CIT1 produces a shift in the ratio of these two activities towards greater methylcitrate synthase activity. 13C-NMR spectroscopic analysis of pyruvate metabolism revealed an accumulation of acetate and isobutanol in &Deltacit3 but not in wild-type or &Deltacit1. These data indicate that Cit3p is the methylcitrate synthase in Saccharomyces cerevisiae. |
| Permanent Link: |
http://rave.ohiolink.edu/etdc/view?acc_num=miami1157995234
http://hdl.handle.net/2374.OX/18486 |
| Date: | 2006 |
Files in this item
| Files | Size | Format | View |
|---|---|---|---|
|
There are no files associated with this item. |
|||