Phylogenetic Analysis of the Heterocystous Cyanobacteria as Assessed by 16S and 23S Ribosomal RNA

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Title:	Phylogenetic Analysis of the Heterocystous Cyanobacteria as Assessed by 16S and 23S Ribosomal RNA
Author:	Kenyon, Kyle Christopher
Description:	This study used 16S and 23S rRNA sequence data for phylogenetic analysis of the heterocystous cyanobacteria. Twenty-eight strains of cyanobacteria were cultured in the laboratory; DNA was extracted; the 16S and 23S ribosomal RNA genes amplified by PCR, cloned, and sequenced. Parsimony, distance and maximum likelihood analysis of the 16S and 23S data supports the monophyly of the heterocystous cyanobacteria. Subsection V cyanobacteria are also supported as a monophyletic clade, embedded within Subsection IV. Members of the genera Calothrix, Tolypothrix and Rivularia are paraphyletic and their relationship is not clearly defined. The Nostoc and Anabaena are not monophyletic or sister to one another. Based on these analyses Anabaena PCC7120 should be reassigned as a Nostoc and PCC73102 Nostoc is not well supported as a type strain for the genus. Nodularia and Cylindrospermum strains are well-supported clades. Two distinct copies of the 23S rRNA were detected in several strains.
Permanent Link:	http://rave.ohiolink.edu/etdc/view?acc_num=miami1060270882 http://hdl.handle.net/2374.OX/18493
Date:	2003

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