EFFECTS OF TCDD ON THE LEVELS OF BIOGENIC AMINES IN RAT BRAINS AFTER SUBCHRONIC EXPOSURE

 
 
 
 

EFFECTS OF TCDD ON THE LEVELS OF BIOGENIC AMINES IN RAT BRAINS AFTER SUBCHRONIC EXPOSURE

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dc.contributor.advisor Byers, James P. en_US
dc.contributor.author Masters, Karilane L. en_US
dc.date.accessioned 2008-07-10T17:59:26Z
dc.date.available 2008-07-10T17:59:26Z
dc.date.created 2004 en_US
dc.date.issued 2008-07-10T17:59:26Z
dc.identifier.uri http://rave.ohiolink.edu/etdc/view?acc_num=toledo1083853239 en_US
dc.identifier.uri http://hdl.handle.net/2374.OX/19416
dc.description It has been recently documented that subchronic exposure of rats to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)causes oxidative damage in various brain regions. In this study, we have investigated the effects of TCDD on the levels of biogenic amines in the rat brain after subchronic exposure. The rats were exposed to TCDD for 13 weeks at 2 different concentrations (22 and 46 ng/kg/day). At the end of the exposure period the animals were euthanized and brain tissue samples were collected from 4 different brain regions: Hippocampus (H), Cerebral Cortex (Cc), Midbrain (MB), and Cerebellum (C). These samples were kept at -80oC. These tissue samples were analyzd for different biogenic amines including NE, DA, DOPAC, 5-HIAA, 5-HT and HVA using a high performance liquid chromatography system with an electrochemical detector. The results show significant increases in DA, DOPAC and HVA in the hippocampus and cerebral cortex but not in the midbrain or the cerebellum in response to the 22 and 46 ng/kg/day doses. However, NE was significantly increased in the hippocampus and cerebral cortex in rats treated with 46 ng/kg/day, it was also increased in the midbrain and cerebellum at both the 22 and 46 ng/kg/day doses. These results are important since many of the TCDD-induced neurotoxic effects in humans are associated with functions of the brain regions found to be affected in this study. en_US
dc.format application/pdf en_US
dc.format 62p. en_US
dc.rights unrestricted en_US
dc.rights Copyright and permissions information available at the source archive en_US
dc.subject TCDD en_US
dc.subject Biogenic amines en_US
dc.subject HPLC en_US
dc.subject High performance liquid chromatography en_US
dc.title EFFECTS OF TCDD ON THE LEVELS OF BIOGENIC AMINES IN RAT BRAINS AFTER SUBCHRONIC EXPOSURE en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name MS en_US
dc.degree.level masters en_US
dc.degree.discipline Pharmaceutical Science en_US
dc.degree.grantor University of Toledo en_US
dc.contributor.publisher University of Toledo / OhioLINK en_US

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