QTL analysis of ray pattern in Caenorhabditis elegans recombinant inbred lines

 
 
 
 

QTL analysis of ray pattern in Caenorhabditis elegans recombinant inbred lines

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Title: QTL analysis of ray pattern in Caenorhabditis elegans recombinant inbred lines
Author: Guess, Adam Joseph
Description: Ray pattern is the most divergent morphological character among rhabditid nematodes. The ancestral ray pattern of Elegans group nematodes is commonly referred to as a 2(1)3+3 pattern, with rays 1 and 2 located anterior of the cloaca, ray 3 positioned at the cloaca, and rays 4-6 and 7-9 grouped in two clusters posterior of the cloaca. C. elegans males primarily exhibit the 2(1)3+3 pattern; however some isolates display variant patterns at a low frequency. The Hawaiian strain CB4856 exhibits a 3/3+3 pattern at a frequency of 0.09. In this pattern, ray 3 is located anterior of the cloaca in a cluster with rays 1 and 2. The designated wild-type strain, N2, is fixed for the 2(1)3+3 pattern. To examine the genetic basis of this phenotypic variation, recombinant inbred lines (RIL) derived from a ten-generation intercross between strains N2 and CB4856 were scored for ray pattern. Transgressive segregation was observed with the 3/3+3 ray pattern being expressed at much higher frequencies in many RIL than in either parental strain. QTL analyses of ray pattern data revealed a single highly significant QTL on the left side of chromosome V (LOD score = 14.273; p = 1.02e-10) as well as a marginally significant QTL on the right side of chromosome I (LOD score = 3.017; p = 0.109). To confirm the major QTL, CB4856 alleles from the left end of chromosome V were introgressed into an otherwise N2 background. From these data, a 500 Kb region of chromosome V was defined that has a major impact on the frequency of the 3/3+3 ray pattern.
Permanent Link: http://rave.ohiolink.edu/etdc/view?acc_num=wright1205197070
http://hdl.handle.net/2374.OX/19504
Date: 2008

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