Home
Tags
No Tags
Measurement of Iso-orotate Decarboxylase Activity in Neurospora Crassa
| Title: | Measurement of Iso-orotate Decarboxylase Activity in Neurospora Crassa |
| Author: | Marshall, Eva M. |
| Description: | The biosynthesis of uridine-5'-monophosphate (UMP) can occur through two pathways. The first and most common is the de novo pathway, while the second and more unique pathway is the thymidine salvage pathway. The fungus Neurospora crassa, unlike many organisms, contains both but utilizes the second of these pathways to convert thymidine to uracil. My research focuses on the fourth enzyme of the thymidine salvage pathway, iso-orotate decarboxylase. Preliminary characterization of IDCase was determined by a radioactivity based assay using 14C to label the substrate IOA. This decarboxylation assay measures the amount of IDCase activity by detecting the amount of radio-labeled CO2 released from IOA in the reaction at a specified time point. |
| Permanent Link: |
http://rave.ohiolink.edu/etdc/view?acc_num=ysu999018116
http://hdl.handle.net/2374.OX/3012 |
| Date: | 1999 |
Files in this item
| Files | Size | Format | View |
|---|---|---|---|
|
There are no files associated with this item. |
|||