FABRICATION AND CHARACTERIZATION OF ELECTROSPUN TECOPHILIC SCAFFOLDS FOR GENE DELIVERY

 
 
 
 

FABRICATION AND CHARACTERIZATION OF ELECTROSPUN TECOPHILIC SCAFFOLDS FOR GENE DELIVERY

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dc.contributor.advisor Yun, Yang H. en_US
dc.contributor.author Williams, Tasha R. en_US
dc.date.accessioned 2008-07-07T02:17:58Z
dc.date.available 2008-07-07T02:17:58Z
dc.date.created 2007 en_US
dc.date.issued 2008-07-07T02:17:58Z
dc.identifier.uri http://rave.ohiolink.edu/etdc/view?acc_num=akron1193882087 en_US
dc.identifier.uri http://hdl.handle.net/2374.OX/3767
dc.description An effective gene therapy wound healing device should have characteristics similar to the extracellular matrix (ECM). To mimic the ECM, delivery devices should be permeable, fibrous structures capable of releasing DNA to cells for transfection. Therefore, plasmid DNA (pDNA) and linear polyethylenimine complexed pDNA (LPEI-pDNA) loaded electrospun Tecophilic® scaffolds are examined as a device for pDNA release. Scanning electron microscopy (SEM) micrographs reveal scaffolds with various morphologies and fiber diameters in the range of 0.5 - 3 μm. Absorbency studies in buffer demonstrate both a mass increase and area increase ratio of two. Tensile studies indicate that the scaffolds are capable of withstanding an ultimate stress of approximately 8 MPa and ultimate strain of ≥ 800 %. In addition, scaffolds are shown to have minimal cellular adhesive properties and are found to be non-cytotoxic to human dermal fibroblast cells in vitro. Agarose gel electrophoresis determines that the encapsulated pDNA retains its structural integrity throughout the fabrication and release processes. Sustained pDNA release is confirmed using PicoGreen® and released pDNA bioactivity is confirmed using fibroblast cells that are successfully transfected in vitro. Electrospun pDNA-Tecophilic® scaffolds are proven advantageous as a device for gene therapy wound healing. Similar to both the ECM and ideal dressings, the scaffolds are strong, elastic, air permeable, minimally adhesive, and non-cytotoxic. When used as a wound dressing, the absorptive properties of the scaffold should help to maintain a moist healing environment. Furthermore, electrospun Tecophilic® scaffolds will provide a sustained localized delivery of pDNA for cellular transfection. en_US
dc.format application/pdf en_US
dc.format 142p. en_US
dc.rights unrestricted en_US
dc.rights Copyright and permissions information available at the source archive en_US
dc.subject DNA en_US
dc.subject DNA Release en_US
dc.subject Electrospinning en_US
dc.subject Gene Delivery en_US
dc.subject Gene Therapy en_US
dc.subject Scaffold en_US
dc.subject Tecophilic en_US
dc.subject Wound Dressing en_US
dc.title FABRICATION AND CHARACTERIZATION OF ELECTROSPUN TECOPHILIC SCAFFOLDS FOR GENE DELIVERY en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name MS en_US
dc.degree.level masters en_US
dc.degree.discipline Biomedical Engineering en_US
dc.degree.grantor University of Akron en_US
dc.contributor.publisher University of Akron / OhioLINK en_US

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