Molecular Mechanism of Incorporation of Factor Va into Prothrombinase

 
 
 
 

Molecular Mechanism of Incorporation of Factor Va into Prothrombinase

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dc.contributor.advisor Kalafatis, Dr. Michael en_US
dc.contributor.author Barhoover, Melissa en_US
dc.date.accessioned 2008-07-07T16:04:33Z
dc.date.available 2008-07-07T16:04:33Z
dc.date.created 2007 en_US
dc.date.issued 2008-07-07T16:04:33Z
dc.identifier.uri http://rave.ohiolink.edu/etdc/view?acc_num=csu1197570167 en_US
dc.identifier.uri http://hdl.handle.net/2374.OX/4084
dc.description The mainstay of the blood coagulation cascade is the formation of the fibrin clot, catalyzed by the serine protease, thrombin. The prothrombinase complex catalyzes the activation of prothrombin to thrombin and, is composed of the enzyme, factor Xa, and the protein cofactor, factor Va, in the presence of divalent metal ions associated on a membrane surface. The enzyme, factor Xa, alone can activate prothrombin by two sequential proteolytic cleavages at Arg271 and Arg320 resulting in the intermediates, Fragment 1.2 and Prethrombin 2. The overall rate of this reaction is not compatible with survival. On the other hand, the incorporation of an excess of the cofactor, factor Va, into prothrombinase reverses the order of the proteolytic cleavages and increases the catalytic activity of factor Xa by 5 orders of magnitude, making this reaction compatible with survival. The goal of this research is to identify the precise amino acids of the central portion of the factor Va heavy chain involved in its incorporation into the prothrombinase complex and the cofactor function it exerts on the catalytic efficiency of prothrombin activation. en_US
dc.format application/pdf en_US
dc.format 157p. en_US
dc.rights unrestricted en_US
dc.rights Copyright and permissions information available at the source archive en_US
dc.subject Factor V en_US
dc.subject Blood Coagulation en_US
dc.subject Prothrombinase en_US
dc.subject APC en_US
dc.subject Thrombin en_US
dc.subject Factor V Leiden en_US
dc.title Molecular Mechanism of Incorporation of Factor Va into Prothrombinase en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name PhD en_US
dc.degree.level doctoral en_US
dc.degree.discipline Science en_US
dc.degree.grantor Cleveland State University en_US
dc.contributor.publisher Cleveland State University / OhioLINK en_US

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