Characterization of changes in hyaluronan following epidermal barrier injury in an organotypic model

 
 
 
 

Characterization of changes in hyaluronan following epidermal barrier injury in an organotypic model

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dc.contributor.advisor Maytin, Edward en_US
dc.contributor.author Ajani, Gati en_US
dc.date.accessioned 2008-07-07T16:04:52Z
dc.date.available 2008-07-07T16:04:52Z
dc.date.created 2008 en_US
dc.date.issued 2008-07-07T16:04:52Z
dc.identifier.uri http://rave.ohiolink.edu/etdc/view?acc_num=csu1209996841 en_US
dc.identifier.uri http://hdl.handle.net/2374.OX/4132
dc.description The stratum corneum, the outermost layer of the epidermis of skin, provides a functional permeability barrier that is essential for our survival and must be rapidly repaired after injury. Of interest to us, a ubiquitous matrix molecule called hyaluronan (HA) has come to the forefront in wound healing research because it is induced after epidermal injury and may be crucial for barrier repair. This thesis examines the role of HA during recovery from the most fundamental form of skin injury, i.e. selective damage to the stratum corneum. The overall question is whether this form of stratum corneum injury can lead to changes in HA similar to that of a full thickness skin wound. To investigate this question, a bioengineered model of barrier injury in organotypic epidermal cultures was first characterized. Changes in HA, in terms of an increase in overall amount and molecular weight, and increased levels of the HA-metabolizing enzymes (HA Synthases 2 and 3) were then measured and defined. Finally, the signalling mechanisms responsible for induction of HA synthesis were explored, revealing that activation of the Epidermal Growth Factor Receptor is essential for the up-regulation of HA after barrier injury. en_US
dc.format application/pdf en_US
dc.format 191p. en_US
dc.rights unrestricted en_US
dc.rights Copyright and permissions information available at the source archive en_US
dc.subject bioengineered model en_US
dc.subject barrier injury en_US
dc.subject hyaluronan en_US
dc.subject epidermis en_US
dc.title Characterization of changes in hyaluronan following epidermal barrier injury in an organotypic model en_US
dc.type Electronic Thesis or Dissertation en_US
dc.degree.name Doctor of Engineering en_US
dc.degree.level doctoral en_US
dc.degree.discipline Fenn College of Engineering en_US
dc.degree.grantor Cleveland State University en_US
dc.contributor.publisher Cleveland State University / OhioLINK en_US

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