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Microarray analysis of drosophila EGF receptor signaling and cell line expression profiles
| Title: | Microarray analysis of drosophila EGF receptor signaling and cell line expression profiles |
| Author: | Butchar, Jonathan P |
| Description: | Despite the enormous advances in our understanding of the Drosophila Epidermal Growth Factor Receptor signaling (dEgfr), it is evident that there are targets of this pathway that remain to be discovered. Here, I employed a microarray-based approach to identify previously undiscovered dEgfr targets in the 3rd -instar wing imaginal disc. Candidate genes were verified in vivo and five were analyzed further. Based on in situ hybridization and functional data, I further hypothesized that one gene, Sulf1, acts in a negative feedback loop to regulate the dEgfr pathway. Genetic interaction crosses confirmed this, and showed that Sulf1 also negatively influences the Notch pathway. Tissue culture experiments using Drosophila cells have been performed for approximately the past 45 years (Brooks and Kurtti, 1971). Despite these decades of research little is known about their transcriptional profiles. Further, it is not yet known exactly which genes are responsible for their taking on an immortalized proliferative state rather than differentiating into their final cell types. To address these issues, I have compared Drosophila cell line groups against a pool of imaginal disc and embryonic datasets. This yielded a list of genes which may be involved with generation and / or maintenance of immortalization. In addition, class prediction and hierarchical clustering routines were used to rapidly differentiate cell lines from one another and to classify a newly created cell line. Results were exported into a database and have been made accessible through a web server. |
| Permanent Link: |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1141824872
http://hdl.handle.net/2374.OX/4250 |
| Date: | 2006 |
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