Identification and characterization of genes involved in the interaction between rice and rice blas fungus, Magnaporthe grisea

 
 
 
 

Identification and characterization of genes involved in the interaction between rice and rice blas fungus, Magnaporthe grisea

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Title: Identification and characterization of genes involved in the interaction between rice and rice blas fungus, Magnaporthe grisea
Author: Jantasuriyarat, Chatchawan
Description: To better understand the molecular basis of the defense response against the rice blast fungus (Magnaporthe grisea), a large-scale expressed sequence tag (EST) sequencing approach was used to identify genes involved in the early infection stages in rice (Oryza sativa). Six cDNA libraries were constructed using infected leaf tissues harvested from 6 conditions: resistant, partially resistant, and susceptible reactions at both 6 and 24 h after inoculation. Two additional libraries were constructed using uninoculated leaves and leaves from the lesion mimic mutant spl11. A total of 68,920 ESTs were generated from 8 libraries. Clustering and assembly analyses resulted in 13,570 unique sequences from 10,934 contigs and 2,636 singletons. A total of 7,748 new and unique ESTs were identified from our collection compared with the KOME full-length cDNA collection. Interestingly, we found that rice ESTs are more closely related to sorghum (Sorghum bicolor) ESTs than to barley (Hordeum vulgare), wheat (Triticum aestivum), and maize (Zea mays) ESTs. Ubiquitination-regulated protein degradation is a common regulatory mechanism that controls a range of cellular processes in eukaryotes. We have recently identified 43 rice ubiquitination-related E2 and E3 genes in our microarray analysis. These genes were induced or suppressed in Pi2 and Pi9 resistant plants after rice blast inoculation. The expression patterns of these E2 and E3 genes were confirmed using northern blot and RT-PCR methods. Among these E2 and E3 genes, we chose the homologue (OsSINAT5) of the Arabidopsis SINAT5 for a detailed functional analysis. Northern blot hybridization and RT-PCR confirmed that OsSINAT5 was induced by rice blast infection as early as 6 hr after inoculation. Functional analysis of this gene in resistance to rice pathogens was conducted using reverse genetics and biochemical approaches.
Permanent Link: http://rave.ohiolink.edu/etdc/view?acc_num=osu1158295749
http://hdl.handle.net/2374.OX/4482
Date: 2006

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